Growing Need for Safe and Effective Guiding Technology During Isolation Drives The Pulsed-Homogeneous Orthogonal Field Gel Electrophoresis (PHOGE) Market
Published by Wanda Rich
Posted on October 7, 2021

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Published by Wanda Rich
Posted on October 7, 2021

In the recent decades, the use of pulsed-field gel electrophoresis (PFGE) in the molecular biology space has been subjected to a lot of research. Pulsed-field gel electrophoresis is a robust tool used to characterize various strains at the DNA level, to obtain useful information on genome size and to create the physical and genetic map of bacteria chromosome that are poorly understood at the genetic level.
Pulsed-field gel electrophoresis is also used in isolating chromosomes in microorganisms, and in the long-range mapping of mammalian genes, and can also examine the extended and oriented configuration of huge DNA molecules in agarose gels at finite field strengths.
To Get In-depth Insights Request for Brochure here –
https://www.factmr.com/connectus/sample?flag=B&rep_id=1129
Genome analysis based on pulsed-field gel electrophoresis has a wide range of applications ranging from typing bacterial strains to radiobiology to cancer research, which could bring some traction in pulsed-field gel electrophoresis market. The methods for running pulsed-field gel electrophoresis have positively changed since its invention, but the method for preparing chromosomal DNA itself is essentially unchanged, which continues to restrict pulsed-field gel electrophoresis applicability, especially when analyses require many samples.
Pulsed-field gel electrophoresis has been an integral part of food safety testing for long, and is still in use today, however it is quickly being replaced. For instance, FSIS (Food Safety and Inspection Service) has recently moved from PFGE to WGS (Whole Genome Sequencing) for analyzing Salmonella and Shiga Toxin-Producing E. coli (STEC) Isolates, as pulsed-field gel electrophoresis doesn’t differentiate isolates to the same degree that can be accomplished by WGS.
Introduced in 1982 by Schwartz et al. Pulsed-field gel electrophoresis (PFGE) is a laboratory technique used by scientists to produce a DNA fingerprint for a bacterial isolate (a group of the same type of bacteria). By comparing the fingerprints of any two isolates, one can investigate if they belong to the same strain (i.e. the two isolates are clonal) or if they are genetically unrelated.
PFGE is a powerful tool for characterizing various strains at the DNA level, obtaining relevant information on genome size and constructing the physical and genetic map of the chromosome of bacteria, in separating chromosomes in microorganisms, and in the long-range mapping of mammalian genes. PFGE is a highly discriminative molecular typing technique based upon the variable migration of large DNA restriction fragments in an electrical field of alternating polarity.
The implementation of PFGE in international surveillance networks, such as PulseNet, and the availability of standardized protocols for important foodborne pathogens (E. coli, Listeria, Campylobacter) have facilitated the adoption of this technique. Though more recent typing methods are available, however, PFGE is still the gold standard in many national and international surveillance programs.
Apart from having advantage of examining the elongated and oriented configuration of large DNA molecules in agarose gels at finite field strengths, PFGE is having various other applications also. Such as, high concordance with epidemiological relatedness, stable and reproducible DNA restriction patterns, and can be applied as a universal generic subtyping method for many different bacteria with only the choice of the restriction enzyme and electrophoresis conditions optimized for each species.
Besides, PFGE is said to be more discriminating than methods such as ribotyping or multi-locus sequence typing for many bacteria. Moreover, PFGE has shown excellent ability to separate small, natural linear chromosomal DNAs ranging in size from 50-kb parasite microchromosomes to multimillion-bp yeast chromosomes. However, intact human chromosomes range in size from 50 million to 250 million bp (Mb), too large for direct PFGE separations.
Pulsed Field Gel Electrophoresis (PFGE) Market: Drivers and Restraints
Primary factors driving Pulsed Field Gel Electrophoresis (PFGE) market include growing need for safe and effective guiding technology during isolation. PulseNet investigates bacterial isolates from sick people, contaminated food, and the places where food is produced. PFGE is the said to be the gold standard fingerprinting method used within PulseNet. However, some limitations of PFGE hindering the growth of pulsed field gel electrophoresis market.
Request Customized Report as Per Your Requirements –
https://www.factmr.com/connectus/sample?flag=RC&rep_id=1129
Pulsed Field Gel Electrophoresis (PFGE) Market: Key Players
The global pulsed field gel electrophoresis systems market is consolidated market and dominated by the are some leading players such as Bio-Rad Laboratories, Inc, Core Life Sciences, Inc., LABREPCO, Analytik Jena AG. Technological advancement in products and expansion of geographical reach are the major trend observed in the global pulsed field gel electrophoresis systems market.
The report is a compilation of first-hand information, qualitative and quantitative assessment by industry analysts, inputs from industry experts and industry participants across the value chain. The report provides in-depth analysis of parent market trends, macro-economic indicators and governing factors along with market attractiveness as per segments. The report also maps the qualitative impact of various market factors on market segments and geographies.
Regional analysis includes
Report Highlights:
Pulsed Field Gel Electrophoresis (PFGE) Market: Segmentation
Tentatively, the global Pulsed Field Gel Electrophoresis (PFGE) market has been segmented on the basis of indication, end users, and geography.
Based on Types, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
Based on applications, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
Enquire Before Buying Here –
https://www.factmr.com/connectus/sample?flag=EB&rep_id=1129
Based on end user, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
Essential Takeaways from the this Market Report
Key questions answered in Pulsed Field Gel Electrophoresis (PFGE) Systems Survey Report:
Explore Fact.MR’s Coverage on the Healthcare Domain:
Digital Therapeutics Market – According to the latest research by Fact.MR, the digital therapeutics market is set to witness exponential growth during 2021-2031. Increasing adoption for digital health to deliver evidence-based therapeutics will witness a promising growth outlook for digital therapeutics in the long run.
Leukocyte Count Testing Market – According to the latest research by Fact.MR, the leukocyte count testing market is set to witness exponential growth with over 7%-8% CAGR during 2021-2031. Increasing bacterial infection with the growing adoption of point of care devices will witness a promising growth outlook for leukocyte count testing in the long run.
Polysaccharide Hemostatic System Market – According to the latest research by Fact.MR, Polysaccharide Hemostatic System market is set to witness a XX% growth during the year 2021-2031.
About Fact.MR
Market research and consulting agency with a difference! That’s why 80% of Fortune 1,000 companies trust us for making their most critical decisions. We have offices in US and Dublin, whereas our global headquarter is in Dubai. While our experienced consultants employ the latest technologies to extract hard-to-find insights, we believe our USP is the trust clients have on our expertise. Spanning a wide range – from automotive & industry 4.0 to healthcare & retail, our coverage is expansive, but we ensure even the most niche categories are analyzed. Reach out to us with your goals, and we’ll be an able research partner.
Contact:
US Sales Office:
11140 Rockville Pike
Suite 400
Rockville, MD 20852
United States
Tel: +1 (628) 251-1583
E-Mail: [email protected]
Corporate Headquarter:
Unit No: AU-01-H Gold Tower (AU),
Plot No: JLT-PH1-I3A,
Jumeirah Lakes Towers,
Dubai, United Arab Emirates
The post Growing Need for Safe and Effective Guiding Technology During Isolation Drives The Pulsed-Homogeneous Orthogonal Field Gel Electrophoresis (PHOGE) Market appeared first on Latest Market Reports.
In the recent decades, the use of pulsed-field gel electrophoresis (PFGE) in the molecular biology space has been subjected to a lot of research. Pulsed-field gel electrophoresis is a robust tool used to characterize various strains at the DNA level, to obtain useful information on genome size and to create the physical and genetic map of bacteria chromosome that are poorly understood at the genetic level.
Pulsed-field gel electrophoresis is also used in isolating chromosomes in microorganisms, and in the long-range mapping of mammalian genes, and can also examine the extended and oriented configuration of huge DNA molecules in agarose gels at finite field strengths.
To Get In-depth Insights Request for Brochure here –
https://www.factmr.com/connectus/sample?flag=B&rep_id=1129
Genome analysis based on pulsed-field gel electrophoresis has a wide range of applications ranging from typing bacterial strains to radiobiology to cancer research, which could bring some traction in pulsed-field gel electrophoresis market. The methods for running pulsed-field gel electrophoresis have positively changed since its invention, but the method for preparing chromosomal DNA itself is essentially unchanged, which continues to restrict pulsed-field gel electrophoresis applicability, especially when analyses require many samples.
Pulsed-field gel electrophoresis has been an integral part of food safety testing for long, and is still in use today, however it is quickly being replaced. For instance, FSIS (Food Safety and Inspection Service) has recently moved from PFGE to WGS (Whole Genome Sequencing) for analyzing Salmonella and Shiga Toxin-Producing E. coli (STEC) Isolates, as pulsed-field gel electrophoresis doesn’t differentiate isolates to the same degree that can be accomplished by WGS.
Introduced in 1982 by Schwartz et al. Pulsed-field gel electrophoresis (PFGE) is a laboratory technique used by scientists to produce a DNA fingerprint for a bacterial isolate (a group of the same type of bacteria). By comparing the fingerprints of any two isolates, one can investigate if they belong to the same strain (i.e. the two isolates are clonal) or if they are genetically unrelated.
PFGE is a powerful tool for characterizing various strains at the DNA level, obtaining relevant information on genome size and constructing the physical and genetic map of the chromosome of bacteria, in separating chromosomes in microorganisms, and in the long-range mapping of mammalian genes. PFGE is a highly discriminative molecular typing technique based upon the variable migration of large DNA restriction fragments in an electrical field of alternating polarity.
The implementation of PFGE in international surveillance networks, such as PulseNet, and the availability of standardized protocols for important foodborne pathogens (E. coli, Listeria, Campylobacter) have facilitated the adoption of this technique. Though more recent typing methods are available, however, PFGE is still the gold standard in many national and international surveillance programs.
Apart from having advantage of examining the elongated and oriented configuration of large DNA molecules in agarose gels at finite field strengths, PFGE is having various other applications also. Such as, high concordance with epidemiological relatedness, stable and reproducible DNA restriction patterns, and can be applied as a universal generic subtyping method for many different bacteria with only the choice of the restriction enzyme and electrophoresis conditions optimized for each species.
Besides, PFGE is said to be more discriminating than methods such as ribotyping or multi-locus sequence typing for many bacteria. Moreover, PFGE has shown excellent ability to separate small, natural linear chromosomal DNAs ranging in size from 50-kb parasite microchromosomes to multimillion-bp yeast chromosomes. However, intact human chromosomes range in size from 50 million to 250 million bp (Mb), too large for direct PFGE separations.
Pulsed Field Gel Electrophoresis (PFGE) Market: Drivers and Restraints
Primary factors driving Pulsed Field Gel Electrophoresis (PFGE) market include growing need for safe and effective guiding technology during isolation. PulseNet investigates bacterial isolates from sick people, contaminated food, and the places where food is produced. PFGE is the said to be the gold standard fingerprinting method used within PulseNet. However, some limitations of PFGE hindering the growth of pulsed field gel electrophoresis market.
Request Customized Report as Per Your Requirements –
https://www.factmr.com/connectus/sample?flag=RC&rep_id=1129
Pulsed Field Gel Electrophoresis (PFGE) Market: Key Players
The global pulsed field gel electrophoresis systems market is consolidated market and dominated by the are some leading players such as Bio-Rad Laboratories, Inc, Core Life Sciences, Inc., LABREPCO, Analytik Jena AG. Technological advancement in products and expansion of geographical reach are the major trend observed in the global pulsed field gel electrophoresis systems market.
The report is a compilation of first-hand information, qualitative and quantitative assessment by industry analysts, inputs from industry experts and industry participants across the value chain. The report provides in-depth analysis of parent market trends, macro-economic indicators and governing factors along with market attractiveness as per segments. The report also maps the qualitative impact of various market factors on market segments and geographies.
Regional analysis includes
Report Highlights:
Pulsed Field Gel Electrophoresis (PFGE) Market: Segmentation
Tentatively, the global Pulsed Field Gel Electrophoresis (PFGE) market has been segmented on the basis of indication, end users, and geography.
Based on Types, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
Based on applications, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
Enquire Before Buying Here –
https://www.factmr.com/connectus/sample?flag=EB&rep_id=1129
Based on end user, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
Essential Takeaways from the this Market Report
Key questions answered in Pulsed Field Gel Electrophoresis (PFGE) Systems Survey Report:
Explore Fact.MR’s Coverage on the Healthcare Domain:
Digital Therapeutics Market – According to the latest research by Fact.MR, the digital therapeutics market is set to witness exponential growth during 2021-2031. Increasing adoption for digital health to deliver evidence-based therapeutics will witness a promising growth outlook for digital therapeutics in the long run.
Leukocyte Count Testing Market – According to the latest research by Fact.MR, the leukocyte count testing market is set to witness exponential growth with over 7%-8% CAGR during 2021-2031. Increasing bacterial infection with the growing adoption of point of care devices will witness a promising growth outlook for leukocyte count testing in the long run.
Polysaccharide Hemostatic System Market – According to the latest research by Fact.MR, Polysaccharide Hemostatic System market is set to witness a XX% growth during the year 2021-2031.
About Fact.MR
Market research and consulting agency with a difference! That’s why 80% of Fortune 1,000 companies trust us for making their most critical decisions. We have offices in US and Dublin, whereas our global headquarter is in Dubai. While our experienced consultants employ the latest technologies to extract hard-to-find insights, we believe our USP is the trust clients have on our expertise. Spanning a wide range – from automotive & industry 4.0 to healthcare & retail, our coverage is expansive, but we ensure even the most niche categories are analyzed. Reach out to us with your goals, and we’ll be an able research partner.
Contact:
US Sales Office:
11140 Rockville Pike
Suite 400
Rockville, MD 20852
United States
Tel: +1 (628) 251-1583
E-Mail: [email protected]
Corporate Headquarter:
Unit No: AU-01-H Gold Tower (AU),
Plot No: JLT-PH1-I3A,
Jumeirah Lakes Towers,
Dubai, United Arab Emirates
The post Growing Need for Safe and Effective Guiding Technology During Isolation Drives The Pulsed-Homogeneous Orthogonal Field Gel Electrophoresis (PHOGE) Market appeared first on Latest Market Reports.