PFGE technology is vital for DNA analysis and food safety, though newer methods like WGS are emerging. It remains key in many surveillance programs.
In the recent decades, the use of pulsed-field gel electrophoresis (PFGE) in the molecular biology space has been subjected to a lot of research. Pulsed-field gel electrophoresis is a robust tool used to characterize various strains at the DNA level, to obtain useful information on genome size and to create the physical and genetic map of bacteria chromosome that are poorly understood at the genetic level.
Pulsed-field gel electrophoresis is also used in isolating chromosomes in microorganisms, and in the long-range mapping of mammalian genes, and can also examine the extended and oriented configuration of huge DNA molecules in agarose gels at finite field strengths.
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Genome analysis based on pulsed-field gel electrophoresis has a wide range of applications ranging from typing bacterial strains to radiobiology to cancer research, which could bring some traction in pulsed-field gel electrophoresis market. The methods for running pulsed-field gel electrophoresis have positively changed since its invention, but the method for preparing chromosomal DNA itself is essentially unchanged, which continues to restrict pulsed-field gel electrophoresis applicability, especially when analyses require many samples.
Pulsed-field gel electrophoresis has been an integral part of food safety testing for long, and is still in use today, however it is quickly being replaced. For instance, FSIS (Food Safety and Inspection Service) has recently moved from PFGE to WGS (Whole Genome Sequencing) for analyzing Salmonella and Shiga Toxin-Producing E. coli (STEC) Isolates, as pulsed-field gel electrophoresis doesn’t differentiate isolates to the same degree that can be accomplished by WGS.
Introduced in 1982 by Schwartz et al. Pulsed-field gel electrophoresis (PFGE) is a laboratory technique used by scientists to produce a DNA fingerprint for a bacterial isolate (a group of the same type of bacteria). By comparing the fingerprints of any two isolates, one can investigate if they belong to the same strain (i.e. the two isolates are clonal) or if they are genetically unrelated.
PFGE is a powerful tool for characterizing various strains at the DNA level, obtaining relevant information on genome size and constructing the physical and genetic map of the chromosome of bacteria, in separating chromosomes in microorganisms, and in the long-range mapping of mammalian genes. PFGE is a highly discriminative molecular typing technique based upon the variable migration of large DNA restriction fragments in an electrical field of alternating polarity.
The implementation of PFGE in international surveillance networks, such as PulseNet, and the availability of standardized protocols for important foodborne pathogens (E. coli, Listeria, Campylobacter) have facilitated the adoption of this technique. Though more recent typing methods are available, however, PFGE is still the gold standard in many national and international surveillance programs.
Apart from having advantage of examining the elongated and oriented configuration of large DNA molecules in agarose gels at finite field strengths, PFGE is having various other applications also. Such as, high concordance with epidemiological relatedness, stable and reproducible DNA restriction patterns, and can be applied as a universal generic subtyping method for many different bacteria with only the choice of the restriction enzyme and electrophoresis conditions optimized for each species.
Besides, PFGE is said to be more discriminating than methods such as ribotyping or multi-locus sequence typing for many bacteria. Moreover, PFGE has shown excellent ability to separate small, natural linear chromosomal DNAs ranging in size from 50-kb parasite microchromosomes to multimillion-bp yeast chromosomes. However, intact human chromosomes range in size from 50 million to 250 million bp (Mb), too large for direct PFGE separations.
Pulsed Field Gel Electrophoresis (PFGE) Market: Drivers and Restraints
Primary factors driving Pulsed Field Gel Electrophoresis (PFGE) market include growing need for safe and effective guiding technology during isolation. PulseNet investigates bacterial isolates from sick people, contaminated food, and the places where food is produced. PFGE is the said to be the gold standard fingerprinting method used within PulseNet. However, some limitations of PFGE hindering the growth of pulsed field gel electrophoresis market.
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Pulsed Field Gel Electrophoresis (PFGE) Market: Key Players
The global pulsed field gel electrophoresis systems market is consolidated market and dominated by the are some leading players such as Bio-Rad Laboratories, Inc, Core Life Sciences, Inc., LABREPCO, Analytik Jena AG. Technological advancement in products and expansion of geographical reach are the major trend observed in the global pulsed field gel electrophoresis systems market.
The report is a compilation of first-hand information, qualitative and quantitative assessment by industry analysts, inputs from industry experts and industry participants across the value chain. The report provides in-depth analysis of parent market trends, macro-economic indicators and governing factors along with market attractiveness as per segments. The report also maps the qualitative impact of various market factors on market segments and geographies.
Regional analysis includes
Report Highlights:
Pulsed Field Gel Electrophoresis (PFGE) Market: Segmentation
Tentatively, the global Pulsed Field Gel Electrophoresis (PFGE) market has been segmented on the basis of indication, end users, and geography.
Based on Types, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
Based on applications, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
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Based on end user, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
Essential Takeaways from the this Market Report
Key questions answered in Pulsed Field Gel Electrophoresis (PFGE) Systems Survey Report:
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The post Growing Need for Safe and Effective Guiding Technology During Isolation Drives The Pulsed-Homogeneous Orthogonal Field Gel Electrophoresis (PHOGE) Market appeared first on Latest Market Reports.
The article discusses the growing need for pulsed-field gel electrophoresis technology in genome analysis and food safety.
PFGE is crucial for DNA fingerprinting, genome size analysis, and bacterial strain typing, despite newer methods emerging.
PFGE is used in food safety testing, bacterial strain typing, and genome analysis, among other applications.
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